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SLM-246

Sigma-Aldrich

Minimum Essential Medium Eagle/F12 complete medium

MEM/F-12 1:1 mixture, with non-essential amino acids, ʟ-glutamine and 10% US origin FBS, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.71
Pricing and availability is not currently available.

Product Name

MEM / F12 Complete Medium, with NEAA, 2mM L-Glut and 10% FBS, 1:1 mixture of MEM medium and Ham′s F12 medium with NEAA, 2mM L-glutamine and 10% U.S. origin FBS

Quality Level

300

form

liquid

technique(s)

cell culture | mammalian: suitable

Quality

pH= 7.2-7.5
Osmolality= 281-312
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= SH-SY5Y Human Neuroblast

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. Can MEM / F12 Complete Medium, with NEAA, 2mM L-Glut and 10% FBS be used for SH-SY5Y cell line?

    1 answer

    1. There are currently two SHSY 5Y cell line products available. DMEM/F12 is the recommended medium for each line. However, each line has been optimized using specific media and reagents. See below for the recommended culture conditions for each cell line:

      SCC291, SHSY-5Y Tet-On Inducible a-Synuclein Neuroblastoma Cell Line
      https://www.emdmillipore.com/product/SHSY-5Y-Tet-On-Inducible-Alpha-Synuclein-Neuroblastoma-Cell-Line,MM_NF-SCC291#documentation
      Medium – DF042, EmbryoMax DMEM/F12
      Serum – ES009-M, Fetal Bovine Serum, stem cell tested

      94030304, SH-SY5Y Cell Line, Human, ECACC
      https://www.sigmaaldrich.com/product/sigma/cb_94030304
      Medium - Ham's F12:EMEM (EBSS) N4888:M2279 (1:1) + 2mM Glutamine (G7513) + 1% Non-Essential Amino Acids (NEAA) (M7145) + 15% Foetal Bovine Serum (FBS) (F2442).
      https://www.sigmaaldrich.com/en/product/sigma/n4888
      https://www.sigmaaldrich.com/en/product/sigma/m2279
      https://www.sigmaaldrich.com/en/product/sigma/g7513
      https://www.sigmaaldrich.com/en/product/sigma/m7145
      https://www.sigmaaldrich.com/en/product/sigma/f2442

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