MABE1867

Anti-CLOCK Antibody, clone CLSP4

• Synonym(s): Circadian locomoter output cycles protein kaput, mCLOCK
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: CLSP4, monoclonal
• mol wt: calculated mol wt 96.39 kDa; observed mol wt ~N/A kDa
• purified by: using protein G
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat
• packaging: antibody small pack of 100
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Internal
• Protein ID accession no.: NP_001276755
• UniProt accession no.: O08785
• storage temp.: 2-8°C
• Gene Information: mouse ... Clock(12753)

Description

Specificity

Clone CLSP4 is a mouse monoclonal antibody that detects CLOCK protein. It targets an epitope within the internal region.

Immunogen

MBP-tagged recombinant fragment corresponding to 180 amino acids from the internal region of mouse CLOCK.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in U2OS cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CLOCK in U2OS cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected CLOCK in Immunofluorescence applications (Umemura, Y., et al. (2017). Proc Natl Acad Sci USA. 114(36):E7479-E7488).

Western Blotting Analysis: A representative lot detected CLOCK in Western Blotting applications (Umemura, Y., et al. (2017). Proc Natl Acad Sci USA. 114(36):E7479-E7488).

Immunoprecipitation Analysis: A representative lot detected CLOCK in Immunoprecipitation applications (Yoshitane, H., et al. (2009). Mol Cell Biol. 29(13):3675-86).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected CLOCK in Immunohistochemistry applications (Umemura, Y., et al. (2017). Proc Natl Acad Sci USA. 114(36):E7479-E7488).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Circadian locomoter output cycles protein kaput (UniProt: O08785; also known as mCLOCK; EC: 2.3.1.48) is encoded by the Clock gene (Gene ID: 12753) in murine species. CLOCK acts as a transcriptional activator that forms a core component of the circadian clock. CLOCK contains 2 PAS domains (aa 107-177 Pas1 domain; aa 262-332 Pas2 domain) and has a nuclear localization signaling sequence (aa 32-47). The major components of circadian clock include CRY proteins, CLOCK or NPAS2, ARNTL/BMAL1 or ARNTL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Transcription and translation of core clock components play a critical role in rhythm generation, whereas delays imposed by post-translational modifications are important for determining the period (tau) of the rhythms. CLOCK forms a heterodimer with ARNTL/BMAL1 and this heterodimer is required for E-box-dependent transactivation and for CLOCK nuclear translocation and degradation. It is also essential for phosphorylation of both CLOCK and ARNTL/BMAL1. CLOCK has an intrinsic acetyltransferase activity, which enables circadian chromatin remodeling by acetylating histones and non-histone proteins, including ARNTL/BMAL1. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 are reported to form the positive limb of the feedback loop, and as a heterodimer activate the transcription of core clock genes and clock-controlled genes. PER1/2/3 and CRY1/2 act as transcriptional repressors and form the negative limb of the feedback loop and inhibit transcription activation. CLOCK is expressed equally in brain, eye, testes, ovaries, liver, heart, lung, and kidney. In the brain, expression is abundant in the suprachiasmatic nuclei, pyriform cortex, and in the hippocampus. (Ref.: Umemura, Y., et al. (2017). Proc. Natl Acad. Sci. USA. 114(36); E7479-E7488; Yoshitane, H., et al. (2009). Mol. Cell Biol. 29(13); 3675-3686).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable