MABD76
Anti-Oct-4 Antibody, clone 9B7
clone 9B7, from mouse
Synonym(s):
POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9B7, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
human (immunogen homology)
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... POU5F1(5460)
General description
The POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
Immunogen
Recombinant protein corresponding to human Oct-4.
Application
Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.
Target description
~39 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse embryonic stem cell lysate
Mouse embryonic stem cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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C H Cole Sims et al.
Stem cell research, 52, 102238-102238 (2021-02-22)
Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation
Samuel C Wolff et al.
Molecular systems biology, 14(9), e8140-e8140 (2018-09-05)
It is well known that clonal cells can make different fate decisions, but it is unclear whether these decisions are determined during, or before, a cell's own lifetime. Here, we engineered an endogenous fluorescent reporter for the pluripotency factor OCT4
Yaser Atlasi et al.
Nature communications, 11(1), 1617-1617 (2020-04-03)
Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. Here, we used genome-wide approaches combined with time-course analysis to measure the mRNA-abundance, mRNA-translation rate and protein expression during
Wiktoria Maria Suchorska et al.
Stem cell reviews and reports, 13(2), 299-308 (2016-12-18)
Stem cells (SCs) are a promising approach to regenerative medicine, with the potential to treat numerous orthopedic disorders, including osteo-degenerative diseases. The development of human-induced pluripotent stem cells (hiPSCs) has increased the potential of SCs for new treatments. However, current
A C Hepburn et al.
Oncogene, 38(22), 4412-4424 (2019-02-12)
Stem cell characteristics have been associated with treatment resistance and poor prognosis across many cancer types. The ability to induce and regulate the pathways that sustain these characteristic hallmarks of lethal cancers in a novel in vitro model would greatly
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