MAB312
Anti-A2B5 Antibody, clone A2B5-105
clone A2B5-105, Chemicon®, from mouse
Synonym(s):
Neuron Cell Surface Antigen
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
A2B5-105, monoclonal
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
isotype
IgM
shipped in
wet ice
target post-translational modification
unmodified
Specificity
Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).
Immunogen
Embryonic chicken retinal cells
Application
Detect A2B5 using this Anti-A2B5 Antibody, clone A2B5-105 validated for use in FC, IC, IF, IH.
Indirect immunofluorescence (1:100 - 1:500) in the detection of neurons in tissue culture.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Physical form
Format: Purified
Liquid in 0.02M PB, 0.25M NaCl, pH 7.6 containing 0.1% sodium azide.
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Type II astrocytes, human neural progenitors
Type II astrocytes, human neural progenitors
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Noah M Walton et al.
Development (Cambridge, England), 133(18), 3671-3681 (2006-08-18)
The isolation and expansion of human neural cell types has become increasingly relevant in restorative neurobiology. Although embryonic and fetal tissue are frequently envisaged as providing sufficiently primordial cells for such applications, the developmental plasticity of endogenous adult neural cells
Sebastián L Vega et al.
Experimental cell research, 351(1), 11-23 (2016-12-31)
Stem and progenitor cells that exhibit significant regenerative potential and critical roles in cancer initiation and progression remain difficult to characterize. Cell fates are determined by reciprocal signaling between the cell microenvironment and the nucleus; hence parameters derived from nuclear
Jeremy A Murphy et al.
Investigative ophthalmology & visual science, 52(10), 7771-7777 (2011-08-30)
To characterize the influence of endothelin-1 (ET-1) on optic nerve head astrocyte (ONHA) proliferation and Ca²⁺ signaling in ONHAs lacking functional endothelin B (ETB) receptors. ONHAs were isolated from adult wild type (WT) and transgenic spotting lethal (TSL) rats, lacking
Hala Gabr et al.
Cell transplantation, 24(9), 1813-1827 (2014-09-10)
Spinal cord injury (SCI) results in demyelination of surviving axons, loss of oligodendrocytes, and impairment of motor and sensory functions. We have developed a clinical strategy of cell therapy for SCI through the use of autologous bone marrow cells for
Natalie D Bull et al.
Investigative ophthalmology & visual science, 50(9), 4244-4253 (2009-04-10)
Glaucoma is a common neurodegenerative disease for which current therapies are often insufficient; thus, new neuroprotective strategies are an important goal. Stem cells are attracting increasing attention as mediators of neuroprotection, often conferred via the trophic support of injured neurons.
Articles
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
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