Gialpha-1. Strong reactivity with rat (100%), mouse (78%), bovine (74%) and guinea pig (48%). Slight reactivity with human (31%). Specificity confirmed by Western blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.
Immunogen
Rat brain G-proteins purified by the method of Sternweis and Robishaw (Sternweis & Robishaw, 1984).
Application
Anti-G Protein Giα-1 Antibody, clone R4 detects level of G Protein Giα-1 & has been published & validated for use in WB.
Research Category Signaling
Research Sub Category GPCR, cAMP/cGMP & Calcium Signaling
Western blot 1:500 - 1:2000.
Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.
Optimal working dilutions must be determined by end user.
Physical form
Liquid ascites, containing sodium azide as a preservative.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
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The regulation of the serotonin transporter (SERT) by guanine nucleotide-binding protein alpha (Gα) q was investigated using Gαq knockout mice. In the absence of Gαq, SERT-mediated uptake of 5-hydroxytryptamine (5HT) was enhanced in midbrain and frontal cortex synaptosomes, but only
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