The Rex-1 (Zfp-42) gene, which encodes an acidic zinc finger protein, is expressed at high levels in embryonic stem (ES) and F9 teratocarcinoma cells. The Rex-1 promoter is regulated by specific octamer family members in early embryonic cells, and is involved in retinoic acid (RA)-associated reduction in expression. Rex-1 is Involved in self-renewal property of ES cells and may be involved in transcriptional regulation of pluripotency signaling pathways. Rex-1 is also involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency and is required for efficient elongation of TSIX, a non-coding RNA antisense to XIST.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of Human Rex-1.
Application
Detect Rex-1 using this Anti-Rex-1 Antibody validated for use in western blotting & ICC.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Rex-1 in a undifferentiated H9 human embryonic stem cells.
Research Category Stem Cell Research
Research Sub Category Pluripotent & Early Differentiation
Quality
Evaluated by Western Blotting in a undifferentiated H9 human embryonic stem cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rex-1 in undifferentiated H9 human embryonic stem cells.
Target description
~35 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Antigen Affinity Purified
Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control Undifferentiated H9 human embryonic stem cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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