Wright Stain (740-75) is intended for use with peripheral blood smears and bone marrows. No procedures have been developed for G banding. There is a Giemsa solution intended for G banding. Currently, there is no developed procedure for G banding with either Wright, Leishman, or other variations of Romanowsky type staining solutions.
740
Wright Stain
Wright Stain
Harleco®, suitable for hematology
Synonym(s):
Hematology Stain
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About This Item
UNSPSC Code:
41116121
EC Index Number:
200-659-6
Pricing and availability is not currently available.
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form
liquid
Quality Level
manufacturer/tradename
Harleco®
mp
-97.77 °C
density
0.792 g/cm3
λmax
520-530 nm (Eosin)
645-655 nm (Azure)
application(s)
diagnostic assay manufacturing
hematology ((contains Methanol))
shipped in
ambient
storage temp.
15-25°C
General description
As a gold standard in hematology, the Harleco® Wright stain is a Romanowsky neutral dye stain based on the combination of methylene blue azure as the basic dye component and eosin as the acidic dye component. The typical purple color of cell nuclei is due to a molecular interaction between eosin Y and an azure B-DNA complex.
This polychromic stain is widely used to identify acidophilic, basophilic, and neutrophilic granules of leukocytes in peripheral blood and bone marrow smears. For decades, the Harleco Wright stain has been used in clinical diagnostics to characterize red blood cell morphology and to differentiate white blood cell types. The staining result can be influenced by several factors such as pH, buffer composition, fixation, and staining time.
The Harleco Wright stain can be used to stain the following sample materials: fresh, native whole blood or bone-marrow smears and clinical cytology specimens (urine sediment, sputum, smears from fine needle aspiration biopsies (FNAB), rinses, and imprints). It may also be used for G-banding to aid in the analysis of poorly spread metaphases.
This polychromic stain is widely used to identify acidophilic, basophilic, and neutrophilic granules of leukocytes in peripheral blood and bone marrow smears. For decades, the Harleco Wright stain has been used in clinical diagnostics to characterize red blood cell morphology and to differentiate white blood cell types. The staining result can be influenced by several factors such as pH, buffer composition, fixation, and staining time.
The Harleco Wright stain can be used to stain the following sample materials: fresh, native whole blood or bone-marrow smears and clinical cytology specimens (urine sediment, sputum, smears from fine needle aspiration biopsies (FNAB), rinses, and imprints). It may also be used for G-banding to aid in the analysis of poorly spread metaphases.
HARLECO® Polychromic stain for peripheral blood and bone marrow smears. Used for red blood cell morphology and white blood cell differentiation.
Application
HARLECO® Polychromic stain for peripheral blood and bone marrow smears. Used for red blood cell morphology and white blood cell differentiation.
Prepared from dyes certified by the Biological Stain Commission (BSC)
For In-Vitro Diagnostic Use.
For Professional Use.
Polychromic stain for peripheral blood and bone marrow smears. Used for red blood cell morphology and white blood cell differentiation.
For In-Vitro Diagnostic Use.
For Professional Use.
Polychromic stain for peripheral blood and bone marrow smears. Used for red blood cell morphology and white blood cell differentiation.
Legal Information
HARLECO is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Target Organs
Eyes,Central nervous system
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Customers Also Viewed
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Upon receiving Part# 740-75 Wright Stain Solution, what are the recommendations for diluting it in Gurr buffer, and how long will slides typically need to be stained for G-banding purposes?
1 answer-
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At the end of the protocol for using the Wright Stain Cat. 740 on bone marrow smears, can mounting medium and coverslip be applied?
1 answer-
The standard advice for peripheral blood and bone marrow smears is not to coverslip. The instructions is to air dry the slides and examining them under a microscope. There is a permanent mounting media with catalog number 64969. The dyes used in Wright Stain will fade with time if a permanent mounting media is used. For this reason, coverslip will not be used. However, paraffin sections from a biopsy that needs to be decalcified and then stained will always be coverslipped.
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