605225

Thrombospondin, Human Platelets

• Synonym(s): Thrombospondin protein
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• description: Merck USA index - 14 9387
• Quality Level: 100
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: soluble
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Native thrombospondin purified from human platelets. Adhesive glycoprotein released in response to platelet activation by α-thrombin. An angiogenesis inhibitor that blocks the FGF-induced neovascularization of the rat cornea. An inducer of platelet aggregation. Synthesized by a number of different cells and secreted into the extracellular matrix. Involved in the regulation of cellular proliferation.

Native thrombospondin purified from human platelets. Adhesive glycoprotein released in response to platelet activation by a-thrombin. Inhibits FGF-induced neovascularization on rat cornea. An inducer of platelet aggregation. Synthesized by a number of cells and secreted into the extracellular matrix. Involved in the regulation of cellular proliferation.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 600 mM NaCl, 20 mM Tris, 1 mM CaCl₂, 20% sucrose, pH 7.8.

Preparation Note

Please refer to lot-specific reconstitution volume on the vial label.

Prepared from platelets that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for 1 week at 4°C or for at least 1 month at -70°C. Product is extremely adherent to surfaces; keep frozen (-70°C) before use and use siliconized surfaces to insure product recovery.

Other Notes

Armstrong, L.C., et al. 2002. Mol. Biol. Cell13, 1893.
Michaund, M. and Poyet, O. 1994. Anticancer Res. 14, 1127.
Castle, V.P., et al. 1993. J. Biol. Chem. 268, 2899.
Koch, A.E., et al. 1993. Pathobiology 61, 1.
Tolsma, S.S., et al. 1993. J. Cell Biol.122, 497.
Tuszynski, G.P., et al. 1993. J. Cell Biol. 120, 513.
Schon, P., et al. 1992. Eur. J. Cell Biol. 59, 329.
Good, D.J., et al. 1990. Proc. Natl. Acad. Sci. USA87, 6624.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1