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Sigma-Aldrich

Anti-Lewis x Mouse mAb (P12)

liquid, clone P12, Calbiochem®

Synonym(s):

Anti-Le x, Anti-Lex

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

tissue culture supernatant

antibody product type

primary antibodies

clone

P12, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FUT3(2525)

General description

Purified mouse monoclonal antibody. Recognizes the Lewis x type 2 oligosaccharide chain.
Recognizes the Lewis x carbohydrate chain in normal kidney.
This Anti-Lewis x Mouse mAb (P12) is validated for use in Paraffin Sections for the detection of Lewis x.

Immunogen

Human
fresh (non-cultured) human placenta cells

Application

Paraffin Sections (1:10-1:120)

Warning

Toxicity: Standard Handling (A)

Physical form

Tissue Culture Supernatant

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Normal kidney tissue

Other Notes

For paraffin sections, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. Variables associated with assay conditions will dictate the proper working dilutions.
Ree, H.J., et al. 1991. Cancer67, 1338.
Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive malignancies with a 5-year survival rate of only 9%. Despite the fact that changes in glycosylation patterns during tumour progression have been reported, no systematic approach has been conducted to
Chen Lu et al.
Journal of visualized experiments : JoVE, (63), e3791-e3791 (2012-05-17)
In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. Glycosylation of proteins is the most prevalent post-translational modification found
Sandy R Pernitzsch et al.
Nature communications, 12(1), 4433-4433 (2021-07-23)
The small, regulatory RNA RepG (Regulator of polymeric G-repeats) regulates the expression of the chemotaxis receptor TlpB in Helicobacter pylori by targeting a variable G-repeat in the tlpB mRNA leader. Here, we show that RepG additionally controls lipopolysaccharide (LPS) phase

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