14-766-M

Tie2 (Y1108F) Protein, active, 10 µg

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 300
• mol wt: M_w 42 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_000459
• UniProt accession no.: Q02763; Q02858 (mouse)

Description

General description

Expressed by baculovirus in Sf21 insect cell.

N-terminal 6His-tagged recombinant human Tie2 residues 771-end.

Tyrosine 1108 has been identified as an autophosphorylation site in human Tie2. The Y1106F substitution in murine Tie2 (analogous to Y1108F in human Tie2) has been shown to block cell migration by disrupting binding of Tie2 to the docking protein Dok-R (Jones N., et al., Mol Cell Biol.(2003);23:2658-2668 and Murray B.W., et al., Biochemistry.(2001);40:10243-10253).

Application

Active, N-terminal 6His-tagged recombinant human Tie2 residues 771-end. For use in Kinase Assays.

Research Category
Inflammation & Immunology

Biochem/physiol Actions

• Protein Target: Tie2
• Target Sub-Family:TK

Packaging

Also available in 250μg size-call for pricing and availability and reference catalog number 14-766M when ordering the 250μg size.

Quality

routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)

Physical form

50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Format: Ni²⁺/NTA-agarose

Storage and Stability

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately s

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable