1.14894

Fractogel^® EMD SE Hicap (M)

• Synonym(s): Fractogel^® EMD SE Hicap (M)
• UNSPSC Code: 41115711

Properties

• ligand: (Sulfoethyl)
• Quality Level: 400
• description: strong cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
• sterility: sterile (Caustic Stable)
• product line: Fractogel^®
• form: resin
• specific analyte(s): MAbs
• parameter: 220 cm/hr flow rate; 8 bar max. pressure
• matrix active group: methacrylate
• mean particle size: 40-90 μm
• capacity: 160 mg binding capacity (lysozyme/ml of resin)
• transition temp: flash point 35 °C (calculated)
• application(s): recombinant protein; vaccine development
• separation technique: cation exchange; strong cation exchange
• storage temp.: 2-30°C

Description

General description

Strong cation exchange Fractogel^® resin featuring the tentacle technology, for purification of basic and neutral proteins (e.g. antibodies) and peptides

Features and Benefits

Fractogel^® EMD SE Hicap (M) features include:

• Tentacle technology provides high yield, selectivity, and focused elution
• High loading capacity combined with high purity at lower elution volumes
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Packaging

• 1.14894.0100: Fractogel^® EMD SE Hicap (M) Resin 100ml
• 1.14894.0010: Fractogel^® EMD SE Hicap (M) Resin 10ml
• 1.14894.0500: Fractogel^® EMD SE Hicap (M) Resin 500ml
• 1.14894.5000: Fractogel^® EMD SE Hicap (M) Resin 5L

Plastic bottle

Analysis Note

Appearance: Milky turbid suspension,free from impurities(foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.08 %
Cerium: ≤ 20 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 120 - 160 mg/ml
Functional test (b:a): ≤ 0.20
Functional test: Separation of chymotrypsinogen A and lysozyme

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 95.0 °F
• Flash Point(C): 35 °C