1.04176

Phloxin B (C.I. 45410)

• Empirical Formula (Hill Notation): C₂₀H₂Br₄Cl₄Na₂O₅
• CAS Number: 18472-87-2
• Molecular Weight: 829.63
• MDL number: MFCD00070627
• UNSPSC Code: 41116130
• NACRES: NA.21

Properties

• form: solid
• Quality Level: 200
• potency: 2870 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥80% gravimetric
• pH: 9.7 (20 °C, 1 g/L in H₂O)
• solubility: 100 g/L
• bulk density: 650 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• SMILES string: [Na+].[Na+].Brc1c2c(cc(c1[O-])Br)C4(OC(=O)c5c4c(c(c(c5Cl)Cl)Cl)Cl)c3c(c(c(c(c3)Br)[O-])Br)O2
• InChI: 1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2
• InChI key: OOYIOIOOWUGAHD-UHFFFAOYSA-L

Description

General description

The dry dye Phloxin B (C.I. 45410) for microscopy Certistain^®, is used for the preparation of a staining solution for cytoplasmic staining in histological sections of human origin. It is mainly used in Hemytoxylin and Eosin (H&E) overview staining that is routinely performed in histological laboratories. Eosin and Phloxine bind to the positively charged plasma proteins. The combination of both dyes results in a broader color range in the tissue. As a CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain^®

Analysis Note

Dye content (gravimetrically): ≥ 80 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 546 - 550 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; ethanol 50 %): 930 - 1400
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable