05-1431

Anti-Casein Kinase 2α Antibody, 1AD9

clone 1AD9, from mouse

• Synonym(s): CK2 catalytic subunit alpha, CKII alpha, Casein kinase II subunit alpha, Casein kinase 2, alpha 1 polypeptide, Casein kinase II alpha 1 subunit, Casein kinase II-alpha subunit, Protein kinase CK2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1AD9, monoclonal
• species reactivity: human, mouse, rat
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001895.3
• UniProt accession no.: P68400
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CSNK2A1(1457)

Description

General description

Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.

Specificity

The antibody binds specifically to the catalytic α-subunit of casein kinase2 (CK-2α). The antibody immunoprecipitates a protein with a molecular weight of 44 kD, CK-2α subunit. A 26 kD protein band, the β-subunit of the heterotetrameric holoenzyme α2β2, is coprecipitated with the CK-2α subunit. In western blots with extracts of kidney tissue, the antibody detects various protein bands between 40 and 44 kDa. Using 1 μg/mL of antibody, the lower detection limit for the holoenzyme is 500 ng in Western blot and 100 ng in dot blot; the detection limits for the α-subunit are 300 ng and 60 ng, respectively.

Immunogen

Epitope: Region common to both alpha and alpha′ subunits

Purified recombinant α-subunit of casein kinase 2.

Application

Anti-Casein Kinase 2α Antibody, 1AD9 detects level of Casein Kinase 2α & has been published & validated for use in IC, WB, IH(P) & IP.

Immunoprecipitation: Recommended

Immunocytochemistry: A previous lot of this antibody was used at a dilution of 5 μg/mL (acetone or methanol fix only).
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of Casein Kinase 2 Monoclonal Antibody: S-KB-3 Cells

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
Developmental Signaling

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.

Target description

44 kDa

Linkage

Replaces: 04-1129

Physical form

Format: Purified

Purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate, pH 7.6 with 0.25 M NaCl and 0.1% sodium azide.

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable