100500P
Avanti
E. coli Extract Total
Avanti Research™ - A Croda Brand
Synonym(s):
E. coli Total Lipid Extract
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About This Item
Recommended Products
form
powder
packaging
pkg of 1 × 1 g (100500P-1g)
pkg of 1 × 100 mg (100500P-100mg)
pkg of 5 × 100 mg (100500P-500mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand
lipid type
lipid extracts
shipped in
dry ice
storage temp.
−20°C
General description
This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.
Application
E. coli Extract Total has been used in the preparation of unilamellar vesicles in lipid screening experiments of membrane protein green fluorescent protein (GFP) based fusion proteins and in the reconstitution of proteoliposomes.
Biochem/physiol Actions
The E. coli lipid extract is a combination of 16, 17 and 18 carbon containing phospholipids namely, phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. It is used for preparing supported lipid bilayers. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in identification and taxonomic grouping of any bacterial sample.
Packaging
5 mL Clear Glass Sealed Ampule (100500P-100mg)
5 mL Clear Glass Sealed Ampule (100500P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100500P-1g)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
also commonly purchased with this product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Membrane insertion of small proteins
Protein Secretion, 39-62 (2010)
An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins
Nji E, et al.
Nature Communications, 9(1), 4253-4253 (2018)
The extracellular gate shapes the energy profile of an ABC exporter
Hutter CAJ, et al.
Nature Communications, 10(1), 2260-2260 (2019)
Marijke Brams et al.
eLife, 9 (2020-01-29)
Pentameric ligand-gated ion channels (pLGICs) or Cys-loop receptors are involved in fast synaptic signaling in the nervous system. Allosteric modulators bind to sites that are remote from the neurotransmitter binding site, but modify coupling of ligand binding to channel opening.
Bettina Zens et al.
Methods (San Diego, Calif.), 75, 37-43 (2014-12-03)
Macroautophagy is a major bulk degradation pathway for cytoplasmic material in eukaryotic cells. During macroautophagy, double membrane-bound organelles called autophagosomes are formed in a de novo manner. In the course of their formation autophagosomes capture cytoplasmic material, which is subsequently
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