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SCP0004

Sigma-Aldrich

γ Secretase Inhibitor

≥95% (HPLC)

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About This Item

Empirical Formula (Hill Notation):
C26H41N3O5
Molecular Weight:
475.62
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

Assay

≥95% (HPLC)

form

lyophilized

composition

Peptide Content, ≥85%

storage condition

protect from light

storage temp.

−20°C

Amino Acid Sequence

Z-Leu-Leu-Nle-al

General description

Gamma-secretase is a protein complex containing presenilin, nicastrin, APH-1, and PEN-2. The leupeptin analogue Z-Leu-Leu-Nle-al (N-benzyloxycarbonyl-Leu-Leu-norleucinal; Benzyloxicarbonyl-Leu-Leu-Nle-CHO (LLNle)) and closely related peptides are used as protease and a gamma secretase inhibitors.

Application

γ Secretase Inhibitor has been used to study the suppressive miR-129 functions through inhibition on estrogen receptor 1 (ESR1) and NOTCH signaling in breast cancer stem cells.[1]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
E239154
LS0649

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Cleonice Michelon et al.
Molecular neurobiology, 57(2), 1159-1169 (2019-11-09)
Inflammatory cytokines are related to impaired learning and memory processes in the central nervous system, contributing to the cognitive dysfunction present in sepsis survivors. In sepsis, brain of survivors presented increased deposition of amyloid-beta (Aβ) peptide and this was associated
MiR-129 blocks estrogen induction of NOTCH signaling activity in breast cancer stem-like cells.
Xiao G, et al.
Oncotarget, 8(61), 103261-103261 (2017)
Tao Na et al.
BMC cancer, 20(1), 1064-1064 (2020-11-06)
Immunomodulatory activities of human mesenchymal stromal /stem cells (hMSCs) has been widely recognized as the most critical function of hMSCs for exerting its therapeutic effects. However, the detailed mechanisms responsible for regulating the immunomodulation of hMSCs still remain largely unknown.
Asha Acharya et al.
PloS one, 6(11), e27743-e27743 (2011-11-24)
Aortic valve calcification is the most common form of valvular heart disease, but the mechanisms of calcific aortic valve disease (CAVD) are unknown. NOTCH1 mutations are associated with aortic valve malformations and adult-onset calcification in families with inherited disease. The
Guodong Xiao et al.
Oncotarget, 8(61), 103261-103273 (2017-12-22)
Stem-like cells in tumor group featured the major role in the chemotherapy resistance of breast cancer, and the reduction of stem-like cells helped to perish the tumor when receiving chemotherapy. Smaller stem cells number indicated better therapeutic effect in vitro
View All Related Papers

Questions

  1. What are the reconstitution instructions for this compound? I checked the SDS, COA, and spec sheet. Also what is the CAS#?

    1 answer

    1. The solubility of this product is in DMSO at 1 mg/ml or higher.

      In biological experiments, water is the most preferred solvent. However, several organic compounds are either not soluble in water or degrade rapidly in the presence of moisture. If DMSO is a recommended solvent, it is best to use a fresh stock bottle that is deemed free of any moisture. Any contaminating moisture may accelerate the degradation of the compound in question or may render it insoluble. It is best to make the initial serial dilutions only in DMSO and then add the final diluted sample to your buffer or the incubation medium. The final concentration of DMSO in vivo should not exceed more than 0.1 %.

      Please see the general information about the use of inhibitors in animals and these data sheets from other γ-Secretase Inhibitors from Calbiochem, which may be useful:
      http://www.emdmillipore.com/product/-Secretase-Inhibitor-I-Calbiochem,EMD_BIO-565750#anchor_PDS
      http://www.emdmillipore.com/product/-Secretase-Inhibitor-XX-CAS-209984-56-5-Calbiochem,EMD_BIO-565789#anchor_PDS

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