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MAK123

Sigma-Aldrich

α-Glucosidase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

α-Glucosidase Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders

storage temp.

−20°C

Gene Information

human ... GAA(2548)
mouse ... GAA(14387)
rat ... GAA(367562)

General description

α-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing a(1→4)-linked D-glucose residues with the release of D-glucose.[1] Defects in α-glucosidase have been implicated in Pompe disease and diabetes.[2]

Application

α-Glucosidase Activity Assay Kit has been used to measure the activity of a-glucosidase.[3][4]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of α-glucosidase activity in biological samples.

Principle

In this assay,α-glucosidase activity is determined by a reaction in which α-glucosidase hydrolyzes p-nitrophenyl-α-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the α-glucosidase activity present

Storage Class Code

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
123CF01A20
123CF01A20
123CE03A19
123CE03A19
123CE06A27

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Epigallocatechin gallate affects glucose metabolism and increases fitness and lifespan in Drosophila melanogaster.
Wagner A E, et al.
Oncotarget, 6(31), 30568-30568 (2015)
Structure of human lysosomal acid a-glucosidase?a guide for the treatment of Pompe disease.
Veronique R Z, et al.
Nature Communications, 8(1), 1111-1111 (2017)
Anika E Wagner et al.
Oncotarget, 6(31), 30568-30578 (2015-09-17)
In this study, we tested whether a standardized epigallocatechin-3-gallate (EGCG) rich green tea extract (comprising > 90% EGCG) affects fitness and lifespan as well as parameters of glucose metabolism and energy homeostasis in the fruit fly, Drosophila melanogaster. Following the
Atta-ur-Rahman.
Studies in Natural Products Chemistry, Volume 37 (2017)
Inhibitory effect of rhubarb on intestinal a-glucosidase activity in type 1 diabetic rats.
Hou K J, et al.
Tropical Journal of Pharmaceutical Research, 16(6), 1293-1297 (2017)
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Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. about cat # MAK123, in the the protocol, the samples are incubated either at room temperature or 37°C and then absorbance is measured after 20 min. Please indicate me the incubation time at RT and incubation time at 37C because kinetic is different at T

    1 answer

    1. The calibrator is incubated under the same conditions as the sample, and the activity is calculated relative to the absorbance of the calibrator, so it doesn't matter what temperature is used for incubation.

      Helpful?

  4. Hi, I have a question about cat # MAK123, a-glucosidase activity assay kit, which is sufficient for 100 assays. Once the reagents are opened, can they be used at a later date? eg - am I able to open the kit and run samples over several days?

    1 answer

    1. The component included in this kit may be refrozen for later use until the expiration date. Do not store refrigerated.

      Helpful?

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