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L9125

Sigma-Aldrich

L-Leucine-p-nitroanilide

leucine aminopeptidase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

L-Leucine-4-nitroanilide

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About This Item

Empirical Formula (Hill Notation):
C12H17N3O3
CAS Number:
4178-93-2
Molecular Weight:
251.28
Beilstein:
2218433
EC Number:
224-047-3
MDL number:
MFCD00063681
UNSPSC Code:
12352204
PubChem Substance ID:
24896516
NACRES:
NA.32
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Product Name

L-Leucine-p-nitroanilide, leucine aminopeptidase substrate

Quality Level

200

Assay

≥98% (TLC)

form

powder

solubility

ethanol: 50 mg/mL

storage temp.

2-8°C

SMILES string

CC(C)C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C12H17N3O3/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18/h3-6,8,11H,7,13H2,1-2H3,(H,14,16)/t11-/m0/s1

InChI key

AXZJHDNQDSVIDR-NSHDSACASA-N

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Application

L-Leucine-p-nitroanilide has been used as substrate
  • for the determination of intestinal leucine aminopeptidase activity of juvenile rainbow trout (Oncorhynchus mykiss)[1]
  • in insulin-regulated aminopeptidase inhibition assay[2]
  • for determining exopeptidase activity[3]

Substrates

Substrate for the colorimetric determination of leucine aminopeptidase.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000393416
0000393822
0000361916
0000361917
0000361919

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Effects of fish meal replacement with meat and bone meal using garlic (Allium sativum) powder on growth, feeding, digestive enzymes and apparent digestibility of nutrients and fatty acids in juvenile rainbow trout (Oncorhynchus mykiss Walbaum, 1792)
Esmaeili M, et al.
Aquaculture Nutrition, 23(6), 1225-1234 (2017)
W Bawab et al.
The Biochemical journal, 286 ( Pt 3), 967-975 (1992-09-15)
Aminopeptidase activities were identified in extracts of kidney, ovotestis, head ganglia, heart and haemolymph of Aplysia californica. These enzyme preparations hydrolysed [3H][Leu]enkephalin at the Try-1-Gly-2 bond as determined by h.p.l.c. analysis of cleavage products. In all these tissues, enkephalin-degrading aminopeptidase
A Bernkop-Schnürch et al.
Pharmaceutical research, 14(2), 181-185 (1997-02-01)
Develop and evaluate systems to prevent aminopeptidase N caused enzymatic degradation of perorally administrated peptide drugs. Bacitracin was covalently bound to the unabsorbable carrier matrix poly(acrylic acid) (paa) in order to avoid any dilution effects of the inhibitor in the
Identification of drug-like inhibitors of insulin-regulated aminopeptidase through small-molecule screening
Engen K, et al.
Assay and Drug Development Technologies, 14(3), 180-193 (2016)
Natasa Bozić et al.
Electrophoresis, 26(12), 2476-2480 (2005-05-28)
A general method for detecting leucyl aminopeptidase activity after native polyacrylamide gel electrophoresis (PAGE) in situ is described. The method is based on diazotization of p-nitroaniline, liberated in the polyacrylamide gel by leucyl aminopeptidase action on leucine-p-nitroanilide (LpNA) and subsequent
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