MDCK I

62106, dog kidney, Polarised epithelial

• Synonym(s): MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• UNSPSC Code: 41106514
• eCl@ss: 32190102

Properties

• Product Name: MDCK I, 00062106
• biological source: dog kidney
• growth mode: Adherent
• karyotype: Dog
• morphology: Polarised epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Canine Cocker Spaniel kidney

Cell Line Description

MDCK I - Madin-Darby canine kidney- is a subclone derived from the heterogeneous parent line MDCK (Madin-Darby canine kidney, Sigma Catalogue number. 85011435). MDCK-I cells, also known as "low passage MDCK" are reported to display a high electrical resistance (over 1000 Ohm/cm²) and form a monolayer with characteristics of tight epithelia of the renal collecting duct. Grown on filters, the cells show a cuboidal polarised morphology and express microvillae-like projections on the apical surface. Cultures on glass possess junctions but no polarised or columnar morphology. MDCK-I cells display an adrenalin, vasopressin and prostoglandin E sensitive adenyl cyclase. Please note, these cells were mycoplasma erradicated prior to deposit at ECACC. Epithelial phenotype may be unstable. Incomplete harvesting at confluence may select for smooth-muscle phenotype this is important if cells being used for transepithelial resistance studies.

Application

Virus susceptibility: Influenza virus, vesicular stomatitis virus and many other viruses.

Culture Medium

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000cells/cm² using 0.25% tryspin or trypsin/EDTA; 5% CO₂; 37°C

Other Notes

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