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SH2121

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, DNA

Ubiquitin Ligases (E1,E2,E3)

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About This Item

Código UNSPSC:
41106609
NACRES:
NA.51

Línea del producto

MISSION®

temp. de almacenamiento

−20°C

Información sobre el gen

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Descripción general

Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Otras notas

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 341, Number of Clones: 2081
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Información legal

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Kim Newton et al.
Cancer investigation, 25(6), 502-513 (2007-09-21)
The regulated degradation of cellular proteins by the ubiquitin-proteasome system impacts a range of vital cellular processes in both normal and cancerous cells. An ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3) catalyzes the conjugation of the protein
Timothy Cardozo et al.
BMC biochemistry, 8 Suppl 1, S9-S9 (2007-12-06)
Recently, the ubiquitin proteasome system (UPS) has matured as a drug discovery arena, largely on the strength of the proven clinical activity of the proteasome inhibitor Velcade in multiple myeloma. Ubiquitin ligases tag cellular proteins, such as oncogenes and tumor

Artículos

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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