Saltar al contenido
Merck

SAB4200792

Sigma-Aldrich

Anti-β-Tubulin IV antibody, Mouse monoclonal

clone ONS.1A6, purified from hybridoma cell culture

Sinónimos:

Anti-TUBB4

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

ONS.1A6, monoclonal

Formulario

buffered aqueous solution

mol peso

~55 kDa

reactividad de especies

chicken, bovine, rat, mouse, human

técnicas

ELISA: suitable
immunoblotting: 10-20 μg/mL using human breast cancer MCF7 cell line.
immunofluorescence: 5-10 μg/mL using human foreskin Hs68 cells.
immunohistochemistry: suitable

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... TUBB(203068)
mouse ... Tubb3(22152)

Descripción general

Tubulin is the major building block of microtubules. It possesses an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, Tubulin exists in all cells as a heterodimer of two similar but not identical polypeptides (approx. 55 kDa each), designated α and β, that assembles into microtubules. Both α and β Tubulins undergo post-translational modifications, including acetylation, phosphorylation, detyrosination, polyglutamylation and polyglycylation. For β-tubulin, six evolutionarily conserved isotypes were identified, designated βI-βVI. Their utilization in the same cell type of different species is nearly absolutely conserved with the exception of the sequenced diversed hematopoietic β-tubulin. Since the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules, it is hypnotized that the β-tubulin isotypes contribute to unique functional properties. The most complex pattern of isotype distribution in tissues is seen in the vertebrate β-tubulins. In mammals βI is constitutive and found in most tissues. βII is found in many tissues, but largely in the brain; its synthesis increases in regeneration and development of neurons. βIII is found in the brain and in dorsal root ganglia; it appears to be localized to neurons, where its expression seems to increase during axonal outgrowth. βIV in mammals exists at two subtypes, differing from each other at 10 positions. βIVa is brain specific and βIVb is ubiquitous, but both appear to be constitutive. βV is ubiquitous expressed in a variety of cultured mammalian cells. βVI is apparently restricted to hematopoietic tissues, being expressed in mammalian platelets, spleen, bone marrow and other blood-forming tissues. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport and cytoskeletal functions. Antibodies reacting specifically with α- and β-tubulin isotypes serve as an essential tool in the detection of the presence and functional significance of these molecules in various cellular settings.

Inmunógeno

Synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype IV, conjugated to BSA.

Aplicación

Immunoblotting: a working concentration of 10-20 μg/mL is recommended using human breast cancer MCF7 cell line.
Immunofluorescence: a working concentration of 5-10 μg/mL is recommended using human foreskin Hs68 cells.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.

Otras notas

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico