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Merck

RAB0507

Sigma-Aldrich

Human VEGF-A ELISA Kit

for serum, plasma, cell culture supernatants and urine

Sinónimos:

VEGF-A, Vascular endothelial growth factor A 

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

reactividad de especies

human

envase

kit of 96 wells (12 strips x 8 wells)

técnicas

ELISA: suitable
capture ELISA: suitable

entrada

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

método de detección

colorimetric

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... VEGFA(7422)

Descripción general

The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in serum, plasma, cell culture supernatants and urine.

Inmunógeno

Recombinant Human VEGF-A

Aplicación

For research use only. Not for use in diagnostic procedures.
Human VEGF (vascular endothelial growth factor) ELISA Kit has been used to measure VEGFA levels in human umbilical vascular endothelial cells (HUVECs) incubated with plasma from pre-eclamptic and healthy pregnant women.
Human VEGF-A ELISA Kit has been used to qualify:
  • VEGF in human liver hepatoma cell line PLC-PRF-5,
  • residual cytokines in decellularized meningeal scaffolds (DMS)
  • human periodontal ligament (hPDL) fibroblasts

Acciones bioquímicas o fisiológicas

VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis. Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes. Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease. Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.

Otras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials


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