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RAB0331

Sigma-Aldrich

Human PSA-total ELISA Kit

for serum, plasma, cell culture supernatant and urine

Sinónimos:

Gamma-seminoprotein), Kallikrein-3, Prostate-specific antigen

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 10.24-2500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... KLK3(354)

General description

The Human PSA-total (Prostate Specific Antigen, total) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human PSA-total in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human PSA

Application

For research use only. Not for use in diagnostic procedures.
Human PSA-total ELISA Kit has been used to determine PSA (prostate specific antigen) level in serum of prostate cancer patients and cancer-free patients.

Biochem/physiol Actions

Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa. Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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André Sarmento-Cabral et al.
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Adipose tissue-derived adipokines (i.e., leptin/adiponectin/resistin) play important roles in the regulation of several pathophysiologic processes through the activation of specific receptors. However, although adipokines and their receptors are widely distributed in many tissues and exhibit a clear modulation according to
Karteek Kadimisetty et al.
Analytical methods : advancing methods and applications, 10(32), 4000-4006 (2019-03-25)
Low cost, miniaturized assay platforms that work with small sample volumes, high sensitivity and rapid detection will have high value in future biomolecular diagnostics. Herein we report an automated, 3D printed electrochemiluminescent (ECL) immunoarray integrated with a nanostructured pyrolytic graphite
Mohamed Sharafeldin et al.
Biosensors & bioelectronics, 91, 359-366 (2017-01-06)
Ultrasensitive mediator-free electrochemical detection for biomarker proteins was achieved at low cost using a novel composite of Fe3O4 nanoparticles loaded onto graphene oxide (GO) nano-sheets (Fe3O4@GO). This paramagnetic Fe3O4@GO composite (1µm size range) was decorated with antibodies against prostate specific
Prostate-specific antigen: biochemistry, analytical methods, and clinical application.
Armbruster DA
Clinical Chemistry, 39(2), 181-195 (1993)
Multiple forms of prostate-specific antigen in serum: differences in immunorecognition by monoclonal and polyclonal assays.
Zhou AM
Clinical Chemistry, 39(12), 2483-2491 (1993)
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