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R6750

Sigma-Aldrich

Ribonucleic acid from baker′s yeast (S. cerevisiae)

Sinónimos:

RNA

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About This Item

Número de CAS:
63231-63-0
MDL number:
MFCD00132195
UNSPSC Code:
41106305
NACRES:
NA.51

Quality Level

200

storage temp.

−20°C

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General description

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is the substrate for RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).

Application

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.

Preparation Note

Prepared by a modification of Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sigma-Aldrich

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Sigma-Aldrich

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Kazuya Shirato et al.
Virology journal, 11, 139-139 (2014-08-12)
The first documented case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) occurred in 2012, and outbreaks have continued ever since, mainly in Saudi Arabia. MERS-CoV is primarily diagnosed using a real-time RT-PCR assay, with at least two different genomic targets
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Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Stephanie C Bannister et al.
Biology of reproduction, 81(1), 165-176 (2009-04-10)
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs
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Protocolos

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

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