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Merck

P9424

Millipore

Proteína A – Agarosa

aqueous ethanol suspension

Sinónimos:

Protein A–Agarose

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About This Item

Número MDL:
Código UNSPSC:
41106500
NACRES:
NA.56
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

Staphylococcus aureus

Nivel de calidad

Formulario

aqueous ethanol suspension

clases químicas de analitos

proteins (Immunoglobulins of various mammalian species)

Extensión del etiquetado

~6 mg per mL

técnicas

affinity chromatography: suitable

Matriz

Sepharose 4B Fast Flow

activación de la matriz

cyanogen bromide

unión a la matriz

amino

espaciador de matriz

1 atom

capacidad

≥30 mg/mL binding capacity (human IgG)

temp. de almacenamiento

2-8°C

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Descripción general

Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.[1]

Aplicación

Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Protein A-Sepharose Fast Flow from Staphylococcus aureus has been used:
  • in co-immunoprecipitation assay[2]
  • in immunodepletion[3]
  • to purify IgG from human serum and plasma[3]

Forma física

Suspension in 20% ethanol

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Flam. Liq. 3

Clase de riesgo para el agua (WGK)

WGK 3


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C Macaulay et al.
The Journal of biological chemistry, 270(1), 254-262 (1995-01-06)
During each cell cycle, the nucleus of higher eukaryotes undergoes a dramatic assembly and disassembly. These events can be faithfully reproduced in vitro using cell-free extracts derived from Xenopus eggs. Such extracts contain three major N-acetylglucosaminylated proteins, p200, p97, and
Joyce A Benjamins et al.
Neurology(R) neuroimmunology & neuroinflammation, 6(3), e550-e550 (2019-05-03)
To identify whether factors toxic to oligodendrocytes (OLs), released by B cells from patients with MS, are found in extracellular microvesicles enriched in exosomes. Conditioned medium (Sup) was obtained from cultures of blood B cells of patients with MS and
Yuhua Zhang et al.
Plant physiology, 149(4), 1860-1871 (2009-02-06)
Trehalose-6-phosphate (T6P) is a proposed signaling molecule in plants, yet how it signals was not clear. Here, we provide evidence that T6P functions as an inhibitor of SNF1-related protein kinase1 (SnRK1; AKIN10/AKIN11) of the SNF1-related group of protein kinases. T6P
H Sternlicht et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(20), 9422-9426 (1993-10-15)
A role in folding newly translated cytoskeletal proteins in the cytosol of eukaryotes has been proposed for t-complex polypeptide 1 (TCP1). In this study, we investigated tubulin and actin biogenesis in Chinese hamster ovary (CHO) cells. When extracts of pulse-labeled
Mazhar Adli et al.
Nature protocols, 6(10), 1656-1668 (2011-10-01)
Chromatin immunoprecipitation (ChIP) combined with high-throughput sequencing (ChIP-seq) has become the gold standard for whole-genome mapping of protein-DNA interactions. However, conventional ChIP protocols necessitate the use of large numbers of cells, and library preparation steps associated with current high-throughput sequencing

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