Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

Sinónimos:

Protease inhibitor solution

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número MDL:
MFCD00677817
Código UNSPSC:
12352200
NACRES:
NA.77

Nombre del producto

Cóctel de inhibidores de proteasas, for use in purification of Histidine-tagged proteins, DMSO solution

origen biológico

synthetic

Nivel de calidad

300

Formulario

DMSO solution

solubilidad

water: soluble

temp. de almacenamiento

−20°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Descripción general

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Especificidad

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Aplicación

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

Características y beneficios

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

Componentes

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Cantidad

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Nota de preparación

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Producto relacionado

Referencia del producto
Descripción
Precios

P8465

Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder

P8340

Cóctel de inhibidores de proteasas, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Cóctel de inhibidores de proteasas, for use with fungal and yeast extracts, DMSO solution

P2714

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

P9599

Cóctel de inhibidores de proteasas, for plant cell and tissue extracts, DMSO solution

P1860

Cóctel de inhibidores de proteasas, for use in tissue culture media, DMSO solution

S8820

Comprimidos de inhibidor de proteasas SIGMAFAST™, For General Use

S8830

Comprimidos de cóctel de inhibidores de proteasas SIGMAFAST™ sin EDTA, for use in purification of Histidine-tagged proteins

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

185.0 °F - closed cup

Punto de inflamabilidad (°C)

85 °C - closed cup

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000305631
0000305631
0000226095
0000226094
0000226094

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Z Kurowska et al.
Neuroscience, 256, 456-466 (2013-10-26)
Nogo-A is a transmembrane protein originally discovered in myelin, produced by postnatal CNS oligodendrocytes. Nogo-A induces growth cone collapse and inhibition of axonal growth in the injured adult CNS. In the intact CNS, Nogo-A functions as a negative regulator of
E Boudreau et al.
The EMBO journal, 19(13), 3366-3376 (2000-07-06)
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2 gene, which is required for the stable accumulation of
Ryan McKay et al.
Biotechnology and bioengineering, 114(12), 2883-2895 (2017-07-30)
Probiotics, whether taken as capsules or consumed in foods, have been regarded as safe for human use by regulatory agencies. Being living cells, they serve as "tunable" factories for the synthesis of a vast array of beneficial molecules. The idea
C Rivier et al.
The EMBO journal, 20(7), 1765-1773 (2001-04-04)
In Chlamydomonas reinhardtii, the psaA mRNA is assembled by a process involving trans-splicing of separate transcripts, encoded at three separate loci of the chloroplast genome. At least 14 nuclear loci and one chloroplast gene, tscA, are needed for this process.
Thomas A Leonard et al.
Cell, 144(1), 55-66 (2011-01-11)
Protein kinase C (PKC) isozymes are the paradigmatic effectors of lipid signaling. PKCs translocate to cell membranes and are allosterically activated upon binding of the lipid diacylglycerol to their C1A and C1B domains. The crystal structure of full-length protein kinase
Ver todos los artículos relacionados

Contenido relacionado

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico