P7275
Pyrophosphate reagent
spectrophotometric assay
Sinónimos:
Pyrophosphate detection reagent
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Productos recomendados
Categorías relacionadas
Application
The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts.
Biochem/physiol Actions
The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH.
Packaging
A technical bulletin is included.
Components
ComponentConcentration
Imidazole × HCl, pH 7.4 45 mM
Citrate 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
Bovine Serum Albumin 5 mg/mL
Sugar Stabilizer 5 mg/mL
PPi-PFK 0.5 units/mL
Aldolase 7.5 units/mL
GDH 5 units/mL
TPI 50 units/mL
Imidazole × HCl, pH 7.4 45 mM
Citrate 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
Bovine Serum Albumin 5 mg/mL
Sugar Stabilizer 5 mg/mL
PPi-PFK 0.5 units/mL
Aldolase 7.5 units/mL
GDH 5 units/mL
TPI 50 units/mL
Quality
A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and α-glycerophosphate dehydrogenase.
Principle
Two moles of NADH are oxidized to NAD+ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm.
The reagent can be used to detect 10-100 nmoles of pyrophosphate.
The reagent can be used to detect 10-100 nmoles of pyrophosphate.
Preparation Note
Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
R Viola et al.
Plant physiology, 97(2), 638-643 (1991-10-01)
Pretreatment of discs excised from developing tubers of potato (Solanum tuberosum L.) with 10 millimolar sodium fluoride induced a transient increase in 3-phosphoglycerate content. This was followed by increases in triose-phosphate, fructose 1,6-bisphosphate and hexose-phosphate (glucose 6-phosphate + fructose 6-phosphate
A continuous spectrophotometric assay for argininosuccinate synthetase based on pyrophosphate formation.
W E O'Brien
Analytical biochemistry, 76(2), 423-430 (1976-12-01)
Erick Strauss et al.
The Journal of biological chemistry, 277(50), 48205-48209 (2002-10-10)
Pantothenic acid (vitamin B(5)) is the natural precursor of coenzyme A (CoA), an essential cofactor in all organisms. The pantothenic acid antimetabolite N-pentylpantothenamide inhibits the growth of Escherichia coli with a minimum inhibitory concentration of 2 microm. In this study
William Swinehart et al.
RNA (New York, N.Y.), 26(9), 1094-1103 (2020-05-10)
N6-threonylcarbamoyl adenosine (t6A) is a nucleoside modification found in all kingdoms of life at position 37 of tRNAs decoding ANN codons, which functions in part to restrict translation initiation to AUG and suppress frameshifting at tandem ANN codons. In Bacteria
Guangkai Bian et al.
Metabolic engineering, 42, 1-8 (2017-04-26)
Terpenoids represent the largest family of natural products. Their structural diversity is largely due to variable skeletons generated by terpene synthases. However, terpene skeletons found in nature are much more than those generated from known terpene synthases. Most promiscuous terpene
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico