P5998

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse

~2 mg/mL, clone 35-206, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD07370940
• UNSPSC Code: 12352203
• eCl@ss: 32160410
• NACRES: NA.44

Propiedades

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 35-206, monoclonal
• form: buffered aqueous solution
• species reactivity: human, rat, mouse
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.04-0.08 μg/mL using whole extract of HeLa cell line
• isotype: IgG2b
• UniProt accession no.: P53350
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PLK1(5347) ; mouse ... Plk1(18817) ; rat ... Plk1(25515)

Descripción

General description

Monoclonal Anti- hPlk1 (Polo Like Kinase 1) (mouse IgG2b isotype) is derived from the 35-206 hybridoma produced by the fusion of mouse myeloma cells (PAI) and splenocytes from BALB/c mice immunized with human full length Plk1. Polo-like kinases (Plks) are a family of serine/threonine kinases. The Plk family is structurally and functionally related to the polo gene in Drosophila. Plk1 localizes during mitosis in centrosomes, kinetochores and in the central spindle.

Immunogen

human full length Plk1.

Application

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunofluorescence staining

Biochem/physiol Actions

PLK1 is a serine-threonine kinase that is expressed in mitotic cells and cancers. This kinase regulates mitotic entry by modulating cdc2/cyclin B and is also involved in Nlp-mediated nucleation of microtubules .

Polo Like Kinase 1 (Plk1) has a role in tumor progression. Plk1 is a target of the DNA damage checkpoint on the basis that its activity is inhibited by DNA damage in G2 and mitosis. Moreover, Plk1 was found to bind to the sequence-specific DNA binding domain of p53. This interaction between p53 and Plk1 inhibits the transcriptional activation and the proapoptotic functions of p53.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)