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P2850

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 1

DMSO solution, mixture of inhibitors

Sinónimos:

Phosphatase Inhibitor

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12352200
NACRES:
NA.54
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Nombre del producto

Phosphatase Inhibitor Cocktail 1, DMSO solution

form

DMSO solution

Quality Level

300

shipped in

wet ice

storage temp.

2-8°C

General description

Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.

Application

Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation.[1] It has also been used in western blot analysis.[2][3]

Biochem/physiol Actions

Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.[4]

Quantity

Recommended usage is a 100-fold dilution.

Analysis Note

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F

flash_point_c

87 °C

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Certificados de análisis (COA)

Lot/Batch Number
0000317618
0000317617
0000215517
056M4081V
17160104

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Larger therapeutic window for steroid versus VEGF-A inhibitor in inflammatory angiogenesis: surprisingly similar impact on leukocyte infiltration.
Nakao S, et al.
Investigative Ophthalmology & Visual Science, 53(7), 3296-3302 (2012)
Class III β-tubulin expression and in vitro resistance to microtubule targeting agents.
Stengel C, et al.
British Journal of Cancer, 102(2), 316-316 (2010)
Discovery of protein phosphatase inhibitor classes by biology-oriented synthesis.
Noren M A, et al.
Proceedings of the National Academy of Sciences of the USA, 103(28), 10606-10611 (2006)
X Zhao et al.
Mucosal immunology, 10(6), 1553-1568 (2017-04-13)
Virulent Mycobacterium tuberculosis (Mtb) triggers necrosis in host Mϕ, which is essential for successful pathogenesis in tuberculosis. Here we demonstrate that necrosis of Mtb-infected Mϕ is dependent on the action of the cytosolic Receptor Interacting Protein Kinase 3 (RIPK3) and
Aberrant Wnt/β-catenin signaling in pancreatic adenocarcinoma.
Zeng G, et al.
Neoplasia, 8(4), 279-289 (2006)
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Phosphatase Inhibitor Cocktail

The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

Questions

1–6 of 6 Questions  

  1. At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?

    1 answer

    1. In most circumstannces, the product is used at a 100-fold dilution.  This will inhibit the phosphatases in 5 mg of protein per mL of lysate.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. How stable is Product P2850, Phosphatase Inhibitor Cocktail 1?  Can I freeze it?

    1 answer

    1. This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.

      Helpful?

  4. Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast?

    1 answer

    1. As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.

      Helpful?

  5. What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?

    1 answer

    1. Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A).  Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.

      Helpful?

  6. Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?

    1 answer

    1. Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C).  Simply allow it to melt before use.  This will not affect the product.

      Helpful?

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