N2640

Fosfato de 4-nitrofenilo disodium salt hexahydrate

chromogenic, tablet

• Sinónimos: 4-Nitrofenil fosfato de disodio, 4-Nitrofenil fosfato de sodio hexahydrate, Fosfato de p-nitrofenilo disodium hexahydrate, pNPP disodium hexahydrate
• Fórmula lineal: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• Número de CAS: 333338-18-4
• Peso molecular: 371.14
• Beilstein/REAXYS Number: 5324661
• EC Number: 224-246-5
• MDL number: MFCD00066288
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897580
• NACRES: NA.83

Propiedades

• product name: Fosfato de 4-nitrofenilo disodium salt hexahydrate, tablet
• form: tablet
• Quality Level: 200
• solubility: water: 1 tablet/3 mL, clear, colorless
• storage temp.: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Descripción

General description

Sustrato cromógeno para la determinación de fosfatasas ácidas y alcalinas. La reacción da un producto final soluble que es de color amarillo. La reacción puede detenerse con NaOH 3 N y leerse a 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

Quantity

Contains 15 mg substrate per tablet

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)