MBD0028
Porphyromonas gingivalis FISH probe - ATTO488
Probe for fluorescence in situ hybridization (FISH)
Sinónimos:
POGI
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.51
Productos recomendados
Nivel de calidad
Formulario
liquid
concentración
20 μM
técnicas
FISH: suitable
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Descripción general
Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells.1 Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples.2
FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood3 and tissue4), microbial ecology (solid biofilms 5 and aquatic systems6) and plants7. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control (MBD0034/35) probes, that accompany the specific probe of interest.
Porphyromonas gingivalis probe specifically recognizes P. gingivalis cells. P. gingivalis, is a gram negative bacterium which is an etiologic agent of adult periodontitis, a chronic inflammatory disease characterized by the destruction of the supportive tissue surrounding teeth. Studies have shown that LPS from P. gingivalis plays an important role in this disease.8-11 The association of the oral microbiota, including P. gingivalis, with various pathological states has been reported. These include development of Alzheimer′s disease12, role in oral cancers13, preterm birth14 and rheumatoid arthritis15. FISH technique was successfully used to identify P. gingivalis with the probe in various samples such as pure culture (as described in the figure legends), dental implants16,17 , periapical tooth lesions18, saliva19, brain tissue20, gingival and aortic tissues21, biofilms from voice prostheses22, subgingival biofilm23, aortic wall tissue24, and infected HeLa cells25. Moreover, FISH can be implicated to identify P. gingivalis in tumor tissue26, multispecies biofilm27, multispecies oral biofilms28 and pure culture and buccal epithelial cells29.
FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood3 and tissue4), microbial ecology (solid biofilms 5 and aquatic systems6) and plants7. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control (MBD0034/35) probes, that accompany the specific probe of interest.
Porphyromonas gingivalis probe specifically recognizes P. gingivalis cells. P. gingivalis, is a gram negative bacterium which is an etiologic agent of adult periodontitis, a chronic inflammatory disease characterized by the destruction of the supportive tissue surrounding teeth. Studies have shown that LPS from P. gingivalis plays an important role in this disease.8-11 The association of the oral microbiota, including P. gingivalis, with various pathological states has been reported. These include development of Alzheimer′s disease12, role in oral cancers13, preterm birth14 and rheumatoid arthritis15. FISH technique was successfully used to identify P. gingivalis with the probe in various samples such as pure culture (as described in the figure legends), dental implants16,17 , periapical tooth lesions18, saliva19, brain tissue20, gingival and aortic tissues21, biofilms from voice prostheses22, subgingival biofilm23, aortic wall tissue24, and infected HeLa cells25. Moreover, FISH can be implicated to identify P. gingivalis in tumor tissue26, multispecies biofilm27, multispecies oral biofilms28 and pure culture and buccal epithelial cells29.
Aplicación
Probe for fluorescence in situ hybridization (FISH),recognizes Porphyromonas gingivalis cells
Características y beneficios
- Visualize, identify and isolate Porphyromonas gingivalis cells.
- Observe native P. gingivalis cell populations in diverse microbiome environments.
- Specific, sensitive and robust identification of P. gingivalis in bacterial mixed population.
- Specific, sensitive and robust identification even when P. gingivalis is in low abundance in the sample.
- FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
- Provides information on P. gingivalis morphology and allows to study biofilm architecture.
- Identify P. gingivalis in clinical samples such as, tumor and brain tissues (for example in formalin-fixed paraffin-embedded (FFPE) samples), saliva and oral cavity and medical equipment such as, dental implants and voice prostheses.
- The ability to detect P. gingivalis in its natural habitat is an essential tool for studying host-microbiome interaction.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Érika Gomes Sarmento et al.
Food research international (Ottawa, Ont.), 116, 1282-1288 (2019-02-06)
Probiotics are widely used in the food industry and may affect the oral microbiota. This study aimed to evaluate the effect of petit-suisse plus probiotic on the microbiota of children's saliva. Strawberry flavor petit-suisse cheese plus green banana flour without
Chunrong Chang et al.
Applied microbiology and biotechnology, 103(3), 1393-1404 (2018-11-25)
Mounting evidence suggests a causal relationship between specific bacterial infections or microbial compositions and the development of certain malignant neoplasms. In this study, we performed research through 16S rRNA amplicon sequencing, qPCR and fluorescence in situ hybridization to certify the
Zijian Cheng et al.
Best practice & research. Clinical rheumatology, 31(1), 19-30 (2017-12-10)
There is an epidemiological association between periodontitis and rheumatoid arthritis (RA), which is hypothesised to lead to enhanced generation of RA-related autoantibodies that can be detected years before the onset of RA symptoms. Periodontitis is a common dysbiotic disease; tissue
Claudia Schillinger et al.
PloS one, 7(5), e37583-e37583 (2012-06-02)
The polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. A number of these microorganisms have
Francesca Cavrini et al.
Journal of medical microbiology, 54(Pt 1), 93-96 (2004-12-14)
Treponema denticola and Porphyromonas gingivalis have been identified in atheromatous plaques of two patients suffering from atherosclerosis by PCR and fluorescence in situ hybridization (FISH). The use of the FISH technique suggested that these periodontopathic micro-organisms might be metabolically active
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