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Merck

MAK156

Sigma-Aldrich

Fatty Acid Uptake Kit

sufficient for 100 fluorometric tests

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

storage temp.

−20°C

General description

Fatty acids are critical for many biological processes including energy metabolism and the synthesis of structural cellular components. The facilitated uptake of fatty acids, presumably through fatty acid transport proteins, is thought to be regulated at multiple levels. The dysregulation of fatty acid uptake may contribute to diseases such as obesity, type 2 diabetes, and hepatic steatosis.

Suitability

This kit is suitable for the measurement of fatty acid uptake in cells containing fatty acid transporters

Principle

This kit is provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. This kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate whose uptake into cells result in an increase in fluorescence intensity (λex = 485/λem = 515 nm).

Storage Class

10 - Combustible liquids


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Cell metabolism, 33(5), 1001-1012 (2021-03-11)
Understanding the mechanisms underlying how T cells become dysfunctional in a tumor microenvironment (TME) will greatly benefit cancer immunotherapy. We found that increased CD36 expression in tumor-infiltrating CD8+ T cells, which was induced by TME cholesterol, was associated with tumor progression and

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