L8525

Lipasa from Candida rugosa

lyophilized powder, ≥40,000 units/mg protein

• Sinónimos: Triacilglicerol acilhidrolasa, Triacilglicerol lipasa
• Número de CAS: 9001-62-1
• Comisión internacional de enzimas: 3.1.1.3 (BRENDA, IUBMB)
• EC Number: 232-619-9
• MDL number: MFCD00131509
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: fungus (Candida rugosa)
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥40,000 units/mg protein
• composition: Protein, ≥4.0%
• foreign activity: α-amylase ≤0.05 unit/mg solid; protease ≤0.001 units/mg solid
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Descripción

General description

Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Las lipasas se utilizan industrialmente para la resolución de los compuestos quirales y la producción de biodiésel mediante transesterificación.

Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement.

Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity.

Lipase from Candida rugosa has been used:

• in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites
• in adsorption of proteins in emulsan/alginate beads and in enzyme assays
• to catalyse methanolysis and hydrolysis reactions

Biochem/physiol Actions

Se hidrolizan los triglicéridos, diglicéridos y monoglicéridos (en orden decreciente de velocidad)

Las lipasas catalizan la hidrólisis de los triacilgliceroles en glicerol y ácidos grasos libres.

Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).

Analysis Note

Protein determined by biuret.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)