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Merck
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L2158

Sigma-Aldrich

L-Leucine p-nitroanilide hydrochloride

chromogenic, ≥98% (HPLC), powder

Sinónimos:

H-Leu-pNA

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About This Item

Fórmula empírica (notación de Hill):
C12H17N3O3 · HCl
Número de CAS:
16010-98-3
Peso molecular:
287.74
MDL number:
MFCD00058248
UNSPSC Code:
12352204
PubChem Substance ID:
24896304
NACRES:
NA.28

product name

L-Leucine p-nitroanilide hydrochloride, ≥98% (HPLC)

Quality Level

300

assay

≥98% (HPLC)

form

powder

solubility

water: 25 mg/mL

storage temp.

2-8°C

SMILES string

Cl.CC(C)C[C@H](N)C(=O)Nc1ccc(cc1)N(=O)=O

InChI

1S/C12H17N3O3.ClH/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18;/h3-6,8,11H,7,13H2,1-2H3,(H,14,16);1H/t11-;/m0./s1

InChI key

PFOYXOWPGRWPLS-MERQFXBCSA-N

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Application

L-Leucine p-nitroanilide hydrochloride has been used as a substrate to measure the activity of leucine aminopeptidase (LAP):
  • for spectrophotometric assays
  • in the intestinal samples, such as duodenum, jejunum, and ileum
  • in the hemolymph of Manila clam

Substrates

Substrate for the colorimetric determination of leucine aminopeptidase.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Susan E Ford et al.
Fish & shellfish immunology, 23(2), 280-291 (2006-12-19)
Components of the haemolymph are understood to constitute the internal defense system of bivalve mollusks and their levels are often considered to be indicators of "health"; however, relatively little proof exists of the role that these elements play in the
Arun Kumar Singh et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 16(5), e375-e381 (2012-03-14)
Staphylococcus aureus has emerged as a major drug-resistant pathogen in hospital- and community-acquired infections. Leucine aminopeptidase (LAP) is known to be essential for survival of the bacteria; however the LAP of S. aureus has not been extensively characterized. In this
J Williams et al.
British poultry science, 49(3), 329-339 (2008-06-24)
1. The objective of this experiment was to study two feeding methods, which could potentially act on the gut microflora, the structure and/or the function of the digestive tract and thereby improve the performance of broilers. 2. Four dietary treatments
Characterization of proteolytic enzyme activities in macroalgae
Perez-Llorens JL, et al.
European Journal of Phycology, 38(1), 55-64 (2003)
Arun Kumar Singh et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 16(5), e375-e381 (2012-03-14)
Staphylococcus aureus has emerged as a major drug-resistant pathogen in hospital- and community-acquired infections. Leucine aminopeptidase (LAP) is known to be essential for survival of the bacteria; however the LAP of S. aureus has not been extensively characterized. In this

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