Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

L2011

Sigma-Aldrich

D-Lactic Dehydrogenase from Lactobacillus leichmannii

ammonium sulfate suspension, ≥250 units/mg protein (biuret)

Sinónimos:

Lactate, (R)-Lactate:NAD+ oxidoreductase, D−LDH

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número de CAS:
9028-36-8
Comisión internacional de enzimas:
1.1.1.28 (BRENDA, IUBMB)
Número MDL:
MFCD00130907
Código UNSPSC:
12352204
NACRES:
NA.54

Formulario

ammonium sulfate suspension

Nivel de calidad

200

actividad específica

≥250 units/mg protein (biuret)

actividad extraña

Malic dehydrogenase <0.5% of base activity

temp. de almacenamiento

2-8°C

Descripción general

Research area: Cell Signalling D-lactate dehydrogenase belongs to the D-isomer-specific 2-hydroxyacid dehydrogenase family. Lactate dehydrogenase (LD) is a tetramer consisting of two (H and M) subunits. It has five isozymes (LD1 to LD5) and this composition varies in different tissues. LD1 is abundant in the kidney, heart, and erythrocytes. LD5 is found in the liver and skeletal muscles. LD is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family.

Acciones bioquímicas o fisiológicas

Lactic dehydrogenase (LDH)plays an important role in the glycolytic pathway where it converts pyruvate tolactate by using NAD+ as a co-factor. It is considered animportant molecule that can be used in cancer therapy as it acts as aglycolytic inhibitor. Inhibition of LDH is associated with blocking of aerobicglycolysis in tumour cells.

Definición de unidad

One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Forma física

Suspension in 3.2 M (NH4)2SO4, pH 6.0

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000384463
0000327089
0000384463
0000327089
0000214334

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

H Taguchi et al.
The Journal of biological chemistry, 266(19), 12588-12594 (1991-07-05)
The gene encoding D-lactate dehydrogenase (D-lactate: NAD+ oxidoreductase, EC 1.1.1.28) of Lactobacillus plantarum has been sequenced, and expressed in Escherichia coli cells with an inducible expression plasmid, in which the 5'-noncoding region of the gene was replaced with the tac
Torben Larsen
Analytical biochemistry, 539, 152-157 (2017-11-06)
D-lactic acid in the mammalian body is mainly of microbiological origin and is often located somewhere along the digestive tract. Surgical, extensive re-sectioning of the small bowel may be one of the risk factors for altered balance in the microbiological
D L Zhang et al.
Postgraduate medical journal, 79(928), 110-112 (2003-03-04)
A 12 year old boy presented with 11 episodes of weakness, ataxia, nausea, slurred speech, dehydration, and sometimes severe lethargy bordering on coma. A year previously the boy had small intestinal resections leaving 20 cm of small bowel remaining. D-lactic
Monica Gallo et al.
Frontiers in bioscience (Landmark edition), 20(8), 1234-1249 (2015-05-12)
Despite the intense scientific efforts made, there are still many tumors that are difficult to treat and the percentage of patient survival in the long-term is still too low. Thus, new approaches to the treatment of cancer are needed. Cancer
Clinical utility of lactate dehydrogenase: a historical perspective.
Ishwarlal Jialal et al.
American journal of clinical pathology, 143(2), 158-159 (2015-01-18)
Ver todos los artículos relacionados

Artículos

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico