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Merck

FLE250

Sigma-Aldrich

Firefly Lantern Extract

for ATP determination

Sinónimos:

FLE

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.81

form

solution

packaging

pkg of 250 mg dried lanterns

technique(s)

toxicology assay: suitable

storage temp.

−20°C

General description

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.

Application

Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.

Biochem/physiol Actions

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.

Other Notes

Crude luciferin and luciferase

Reconstitution

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Nausch M, et al.
Frontiers in Marine Science, 5(386) (2018)
Distribution patterns of organic pollutants and microbial processes in marine sediments across a gradient of anthropogenic impact
Zoppini A, et al.
Environmental Pollution (Barking, Essex : 1987), 242, 1860-1870 (2018)
Involvement of acetylcholinesterase and protein kinase C in the protective effect of caffeine against beta-amyloid-induced alterations in red blood cells
Carelli-Alinovi C, et al.
Biochimie, 121, 52-59 (2016)
Jennifer P Richards et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 308(5), R411-R418 (2015-01-02)
The circulating erythrocyte, by virtue of the regulated release of ATP in response to reduced oxygen (O2) tension, plays a key role in maintaining appropriate perfusion distribution to meet tissue needs. Erythrocytes from individuals with Type 2 diabetes (DM2) fail
Microbial responses to polycyclic aromatic hydrocarbon contamination in temporary river sediments: Experimental insights
Zoppini A, et al.
The Science of the Total Environment, 541, 1364-1371 (2016)

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