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F4143

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Número MDL:
MFCD00162628
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

200

conjugado

FITC conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

condiciones de almacenamiento

protect from light

técnicas

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
indirect immunofluorescence: 1:128

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

IgG antibody is secreted by B cells and is found in blood and extracellular fluids and
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system.

Especificidad

Binds mouse IgG; does not bind other mouse Igs.

Inmunógeno

purified mouse IgG, Fc fragment

Aplicación

Anti-Mouse IgG (Fc specific)-FITC antibody has been used in
  • immunohistochemistry
  • immunofluorescence
  • flow cytometry
  • immunocytochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Acciones bioquímicas o fisiológicas

IgG provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Otras notas

Antibody adsorbed with bovine, equine and human serum proteins.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparación

Adsorbed to reduce background staining with bovine, horse or human samples.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000231048
0000225534
0000231048
0000225534
0000207679

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Jiří Zahradník et al.
ACS synthetic biology, 10(12), 3445-3460 (2021-11-24)
Here, we enhanced the popular yeast display method by multiple rounds of DNA and protein engineering. We introduced surface exposure-tailored reporters, eUnaG2 and DnbALFA, creating a new platform of C and N terminal fusion vectors. The optimization of eUnaG2 resulted
P Xu et al.
Iranian journal of veterinary research, 22(1), 65-71 (2021-06-22)
Mammary epithelial cells (MECs) have been widely-used over the years as models to understand the physiological function of mammary disease. This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMECs) from
Qiong Wu et al.
Frontiers in microbiology, 9, 1661-1661 (2018-08-09)
Escherichia coli is a common cause of mastitis in dairy cows. The adaptor protein apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) synergizes with caspase-1 to regulate inflammasome activation during pathogen infection. Here, the ASC gene was knocked out
Natalia G de Isla et al.
Transfusion, 43(8), 1145-1152 (2003-07-19)
The quantification of antigens and proteins on RBCs has been achieved by different approaches. Flow cytometry allows the results of the earliest studies to be to reappraised because it offers the possibility of measuring the immunofluorescence intensity of single cells
Patrick L Sinn et al.
Journal of virology, 77(10), 5902-5910 (2003-04-30)
The practical application of gene therapy as a treatment for cystic fibrosis is limited by poor gene transfer efficiency with vectors applied to the apical surface of airway epithelia. Recently, folate receptor alpha (FR alpha), a glycosylphosphatidylinositol-linked surface protein, was
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