E5134

Ácido etilenodiaminotetraacético disodium salt dihydrate

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

• Sinónimos: EDTA disodium salt, EDTA-Na₂, Edatamil, Edetato disodium salt dihydrate, Etilenodiaminotetraacetato de disodio dihydrate, Sequestrene Na₂
• Fórmula empírica (notación de Hill): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• Número de CAS: 6381-92-6
• Peso molecular: 372.24
• Beilstein/REAXYS Number: 3900609
• EC Number: 205-358-3
• UNSPSC Code: 12352107
• PubChem Substance ID: 24894605
• NACRES: NA.31

Propiedades

• grade: for molecular biology
• assay: 99.0-101.0% (titration)
• form: powder
• reaction suitability: reagent type: chelator
• technique(s): electrophoresis: suitable
• impurities: ≤0.005% Insolubles; ≤0.1% Nitrilotriacetic acid (NTA)
• mp: 248 °C (dec.) (lit.)
• solubility: 3 M NaOH: 1.6 g/10 mL
• cation traces: Fe: ≤0.005%; Pb: ≤0.002%
• suitability: suitable for electrophoresis; suitable for molecular biology
• foreign activity: DNase, RNase, NICKase and protease, none detected
• storage temp.: room temp
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Descripción

General description

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

Application

EDTA disodium salt has been used in research:

• as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
• an anticoagulant for whole blood collection
• a stabilizer solution for freezing cell culture samples
• in combination with PBS and trypsin, to trypsinize cells
• for phytoextraction of heavy metals in polluted soils

Quelante de cationes divalentes. Inhibe las enzimas, como las metaloproteasas, que requieren cationes divalentes para ser activas.

Features and Benefits

• Molecular Biology grade, suitable for many molecular biology applications
• Free of endonucleases DNase, RNase, Nickase, and Protease

Other Notes

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Información de seguridad

• pictograms: GHS07,GHS08
• signalword: Warning
• hcodes: H332 - H373
• pcodes: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• target_organs: Respiratory Tract
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)