E0760

Endothelial cell growth supplement from bovine pituitary

Bioreagent, powder, suitable for cell culture

• Sinónimos: ECGS, Endothelial Mitogen
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Nombre del producto: Endothelial cell growth supplement from bovine pituitary, ECGS, suitable for cell culture, BioReagent
• origen biológico: bovine pituitary glands
• Nivel de calidad: 200
• Línea del producto: BioReagent
• Formulario: powder
• potencia: 2-300 μg/mL ED₅₀/EC₅₀
• calidad: endotoxin tested
• envase: pkg of 15 mg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | mammalian: suitable
• impurezas: ≤20 EU/vial (LAL test)
• color: brown to tan
• solubilidad: water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands[1] containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Aplicación

Endothelial cell growth supplement from bovine pituitary has been used:

• as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture[2]
• as a supplement in medium 199 for endothelial cell culture[3]
• as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12[4]
• as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture[5]

Acciones bioquímicas o fisiológicas

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Warning
• Frases de peligro: H361fd
• Consejos de prudencia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Repr. 2
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable