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E0282

Sigma-Aldrich

MaxGel™ ECM

mixture, liquid

Sinónimos:

Human Basement Membrane Extract (BME)

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About This Item

Código UNSPSC:
12352207
eCl@ss:
32190102
NACRES:
NA.71
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

200

Formulario

liquid

temp. de almacenamiento

−70°C

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Categorías relacionadas

Aplicación

MaxGel™ ECM is used for the following applications:
  • Extracellular matrix (ECM) protein binding assays[1][2]
  • Cell invasion assay[3]
  • Cell culture[4]
This product creates a three-dimensional environment that improves cellular proflieration by promoting cell growth and migration, and has been shown to aid the proliferation of many types of cells, including human embryonic stem cells, neural stem cells, neurons, glia, astrocytes, fibroblasts, hepatocytes, and keratinocytes.

Componentes

MaxGel ECM contains human extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin and a number of proteoglycans and glycosaminoglycans. This basement membrane extract reproduces the cooperative interactions between epithelia and mesenchyme that occur during the development and organotypic cell culture of skin.

Precaución

MaxGel will remain stable for at least 1 year when stored at -70°C.

Información legal

MaxGel is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SLCQ2147
SLCN0499
SLCJ9762
SLCK8873
SLCM8909

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Donna M Easton et al.
Frontiers in microbiology, 2, 120-120 (2011-06-21)
Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) are diarrheagenic pathotypes of E. coli that cause gastrointestinal disease with the potential for life-threatening sequelae. While certain EHEC and EPEC virulence mechanisms have been extensively studied, the factors that mediate
Astrid Subrizi et al.
Biomaterials, 33(32), 8047-8054 (2012-08-16)
The in vitro generation of a functional retinal pigment epithelium (RPE) for therapeutic applications requires a limitless source of RPE cells and a supporting scaffold, which improves cell survival and promotes the acquisition of the RPE phenotype. We successfully differentiated human
Bin He et al.
Oncology reports, 29(3), 1115-1123 (2012-12-21)
LIM and SH3 protein 1 (LASP1) is an actin-binding protein which is overexpressed in many types of cancers and plays important roles in cancer progression. however, the role of LASP1 in esophageal squamous cell carcinoma (ESCC) is still unknown. We sought
J Křivánek et al.
Folia biologica, 60 Suppl 1, 30-38 (2014-11-05)
Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to
H M Sharon Goh et al.
Applied and environmental microbiology, 79(21), 6535-6543 (2013-08-21)
Acinetobacter baumannii is a multidrug-resistant pathogen associated with hospital outbreaks of infection across the globe, particularly in the intensive care unit. The ability of A. baumannii to survive in the hospital environment for long periods is linked to antibiotic resistance
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Questions

1–7 of 7 Questions  

  1. How do I handle Product E0282, MaxGel™ ECM?

    1 answer

    1. Remove the product from the freezer and thaw on ice just prior to use. Keep the product cold until coating.

      Please see the link below to review the product datasheet which includes the coating protocol:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdf

      Helpful?

  2. is the Product E0282, MaxGel™ ECM from normal tissue or tumor tissue?

    1 answer

    1. This product is derived from the basement membranes of epithelial cells and fibroblasts. These are not strictly tumor cell lines as the normal genotype and phenotype are retained.

      Helpful?

  3. Can this product also be used for cell differentiation?

    1 answer

    1. The impact of extracellular matrix on cell differentiation can vary from cell type to cell type. This product is derived from human basement membrane extract that includes several components, many of which are not defined. Cell differentiation may be induced by the structural environment or other components present within the matrix. The end user would have to determine the suitability of the product based on the cell or tissue type of interest.

      Please refer to the literature for additional information. The following link may be of interest:
      https://biosignaling.biomedcentral.com/articles/10.1186/s12964-023-01252-8#:~:text=Several%20studies%20have%20demonstrated%20that,a%20certain%20niche%20%5B46%5D.

      Please see the link below to review the product datasheet for this item:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdf

      Helpful?

  4. What is an appropriate working concentration for Product E0282, MaxGel™ ECM?

    1 answer

    1. MaxGel™ works best undiluted or even diluted 1:2-1:4.  For a thin coat plating method, this material can be diluted as high as 1:100.

      Helpful?

  5. How is Product E0282, MaxGel™ ECM, supplied?

    1 answer

    1. E0282 is supplied at a concentration of 1 mg/ml in DMEM.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. Will Product E0282, MaxGel™ ECM, work for adherent cells and suspension cells?

    1 answer

    1. Yes, this product works for both cell types.

      Helpful?

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