The formulation utilizes Calcium Chloride Anhydrous rather than the dihydrate form.
D5030
Dulbecco′s Modified Eagle′s Medium
Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture
Sinónimos:
DME, DMEM
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nivel de calidad
Formulario
powder
técnicas
cell culture | mammalian: suitable
componentes
phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
Descripción general
Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.
Aplicación
Dulbecco′s Modified Eagle′s Medium has been used:
- to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
- to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
- to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells
Cantidad
Formulated to contain 8.3 grams of powder per liter of medium.
Reconstitución
Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
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también adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Protocolos
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Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.
Contenido relacionado
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
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Is the CaCl2 included in Product D5030 in the form of a dihydrate or is it anhydrous?
1 answer-
Helpful?
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Is there an available variant of EMEM that is free from phenol red, glutamine, and NaHCO3? Additionally, for an MTT assay, would it be necessary to have two types of media – one being the aforementioned EMEM variant and the other for regular culture growth?
1 answer-
For an MTT assay, standard RPMI or DMEM media are suitable options. These include RPMI (Product R0883) and DMEM (Product D5671), both of which may contain up to 10% FBS. When performing assays, such as mitogen or complement-mediated cytotoxicity assays, cells should be suspended in a tissue culture medium, such as DMEM or RPMI, before adding the MTT reagent to each well.
Helpful?
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What are the preparation instructions of the 10L product?
1 answer-
As listed on the product detail page in the 'DESCRIPTION' section under 'Reconstitution', this product can be supplemented with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
Helpful?
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How can we reconstitute this powder to create ready-to-use media? What is the reason for excluding Sodium bicarbonate from powdered media?
How can we reconstitute this powder to create ready-to-use media? What is the reason for excluding Sodium bicarbonate from powdered media?
0 answers
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