D1821

DAF-FM

≥98% (HPLC)

• Sinónimos: 4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM
• Fórmula empírica (notación de Hill): C₂₁H₁₄F₂N₂O₅
• Número de CAS: 254109-20-1
• Peso molecular: 412.34
• Número MDL: MFCD29073049
• Código UNSPSC: 12352204
• ID de la sustancia en PubChem: 329798711
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥98% (HPLC)
• Formulario: powder
• condiciones de almacenamiento: desiccated
• color: white to brown
• solubilidad: DMSO: >1 mg/mL
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• cadena SMILES: CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• Clave InChI: DIJCILWNOLHJCG-UHFFFAOYSA-N

Descripción

Aplicación

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Acciones bioquímicas o fisiológicas

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Precaución

hygroscopic

Nota de preparación

Color variation within the range from white to brown does not change performance.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable