CS0790
Antioxidant Assay Kit
sufficient for 200 tests
Sinónimos:
Oxidative Stress Detection Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Productos recomendados
uso
sufficient for 200 tests
Nivel de calidad
método de detección
colorimetric
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
Free radicals or reactive oxygen species (ROS) are produced during biochemical redox reactions as part of normal physiological cell metabolism (protection from infectious organisms) and as a response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation. Once formed, ROS attack cellular components causing damage to lipids, proteins, and DNA, which can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.
In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant.
Aplicación
Antioxidant assay kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates. It has also been used to measure the radical cation (2,2′-azino-di-[3-ethylbenzthiazoline sulphonate]) (ABTS+) scavenging capacity.
It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva.
Idoneidad
Suitable for the measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates
Principio
The principle of the antioxidant assay is formation of a ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation, ABTS·+ , a soluble chromogen that is green in color and can be determined spectrophotometrically at 405 nm.
Nota de preparación
Reagents are to be prepared with ultrapure water.
Reconstitución
Myoglobin Solutions are to be stored at -20C after reconstitution.
Trolox Working Solution is to be stored at -20C after reconstitution.
ABTS Substrate Solution is to be stored at 4C for 2 weeks or at -20C for 1 month.
Trolox Working Solution is to be stored at -20C after reconstitution.
ABTS Substrate Solution is to be stored at 4C for 2 weeks or at -20C for 1 month.
Información legal
Solo componentes del kit
Referencia del producto
Descripción
- Assay Buffer 10X 30 mL
- Stop Solution 20 mL
- Hydrogen Peroxide, 3% solution 1 mL
- Myoglobin from horse heart 2 x 1
- (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1
- ABTS, 10 mg tablets 4 tab
- Phosphate-Citrate Buffer, pH 5 4 tab
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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