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CS0260

Sigma-Aldrich

Glutathione Assay Kit

sufficient for 700 assays

Sinónimos:

GSH Quantification Test

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
En este momento no podemos mostrarle ni los precios ni la disponibilidad

usage

sufficient for 700 assays

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... GSR(2936)

General description

The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm.

Application

Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay.[1][2][3] It also has been used to determine different oxidative stress parameters in rats.[4]

Biochem/physiol Actions

Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases.[5]
Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues

Features and Benefits

  • A quick and simple method
  • The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)
  • The kit includes a deproteination reagent for biological samples

Solo componentes del kit

Referencia del producto
Descripción
  • Assay Buffer 5x 30 mL
  • DTNB 8 mg
  • DMSO 7.5 mL
  • Glutathine Reductase 20 U
  • L-Glutathione Reduced 300 μg
  • NADPH 25 mg
  • 5-Sulfosalicylic Acid 2.5 g

Related product

Referencia del producto
Descripción
Precios

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

hcodes

H314,H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

188.6 °F

flash_point_c

87 °C

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Certificados de análisis (COA)

Lot/Batch Number
0000253807
0000325609
0000325609
0000253807
099M4064V

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Rat testicular impairment induced by electromagnetic radiation from a conventional cellular telephone and the protective effects of the antioxidants vitamins C and E
Al-Damegh MA
Clinics, 67(7), 785-792 (2012)
Nicol M Caplin et al.
Frontiers in plant science, 11, 389-389 (2020-05-01)
The effects of ionising radiation (IR) on plants are important for environmental protection but also in agriculture, horticulture, space science, and plant stress biology. Much current understanding of the effects of IR on plants derives from acute high-dose studies but
Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples.
T P Akerboom et al.
Methods in enzymology, 77, 373-382 (1981-01-01)
Maria Swastika et al.
Malaria journal, 19(1), 208-208 (2020-06-20)
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzyme disorder in the world. Its main function is to generate NADPH that is required for anti-oxidative pathway in the cells especially in red blood cells (RBC). G6PD deficiency is X-linked and
Genotoxicity of hydroquinone in A549 cells
Peng C, et al.
Cell Biology and Toxicology, 29(4), 213-227 (2013)
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Questions

1–8 of 8 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What is the detection limit of glutathione assay kit?

    1 answer

    1. The assay range for this kit is 3.125 µM to 50.0 µM.

      Helpful?

  4. How can I calculate the portion of GSH and GSSG after I get the total Glutathione in the samples?

    1 answer

    1. This kit is not intended for use in determining GSH:GSSG ratio. It measures both total glutathione (oxidised + reduced GSSG + GSH) as it operates with a DTNB reagent. Please see page 3 of the technical bulletin which shows the operating principle of this kit:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/418/394/cs0260bul.pdf

      Product MAK440 is an alternative kit that measures both GSH and GSSG. See the link below to review this technical bulletin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/146/686/mak440pis-ms.pdf

      Helpful?

  5. Can you measure whole blood glutathione using this kit?

    1 answer

    1. Yes, this kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in cell and tissue extracts and in red blood cells or plasma.

      Refer to this link to know more: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/418/394/cs0260bul.pdf

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. How do I calculate DA412/min when using Product CS0260, Glutathione Assay Kit?

    1 answer

    1. The delta A412/min (sample) stands for the average increase in value per min of the OD over a 4 minute period. The delta A412/min (1 nmole) can be calculated by regression analysis of the points of the standard curve. The standard curve should be obtained by plotting a graph of delta A412/min versus nmole GSH.

      Helpful?

  8. Is Product CS0260, Glutathione Assay Kit, for determination of reduced glutathione, oxidized glutathione, or both?

    1 answer

    1. This kit is designed to measure total glutathione. The glutathione reductase included in the kit will reduce oxidized glutathione present in the sample.

      Helpful?

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