HKT-1097

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: hamster kidney
• envase: tube of 5 μg 98061003-DNA-5UG; pkg of vial of cells 98061003-1VL
• modo de crecimiento: Adherent
• cariotipo: Modal no. 44
• morfología: Fibroblast-like
• productos: Not specified
• receptores: EGF
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Syrian Hamster kidney

Descripción línea celular

The cell line HKT-1097 has been established from a 14 month old male Syrian Hamster (Mesocricetus auratus) kidney tumour which has been induced by diethylstilbestrol exposure. HKT-1097 cells express epidermal growth factor (EGF) receptor, Erb-B2 and Erb-B3 as well as vimentin, desmin, glial fibrillary acidic protein (GFAP), TGF alpha, PGP 9.5 and S100 protein and neuron-specific enolase. Karyotype analysis revealed a modal number of 44 chromosomes (41% of cells) and polyploidy in approximately 2% of cells. The cells have a doubling time of about 36 hours.

Perfil del ADN

Not specified

Medio de cultivo

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4, i.e., seeding at 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Form a multipolar, loosely packed monolayer.

Otras notas

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