KYSE-270

94072021, human esophagus, Epithelial

• Sinónimos: KY-270 Cells, KYSE270 Cells
• UNSPSC Code: 41106514

Propiedades

• product name: KYSE-270, 94072021
• biological source: human esophagus
• growth mode: Adherent
• karyotype: Hypotriploid with 16% polyploidy
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human oesophageal squamous cell carcinoma

Cell Line Description

Established from the well differentiated invasive oesophageal squamous cell carcinoma resected from the middle intra-thoracic oesophagus of a 79-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC.

Culture Medium

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS)

Subculture Routine

Split confluent cultures 1:5 every second day using trypsin/EDTA;5% CO₂; 37°C. Doubling time: 24 hours. Recommended seeding density: 2.5 x 10,000 cells/cm².

Other Notes

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