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PC-9 (formerly known as PC-14)

90071810, human lung, Heterogeneous mixture of round cells and spindle shaped cells

Sinónimos:

PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells

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About This Item

Código UNSPSC:
41106514

Nombre del producto

PC-9 (formerly known as PC-14), 90071810

origen biológico

human lung

modo de crecimiento

Adherent/suspension

cariotipo

Not specified

morfología

Heterogeneous mixture of round cells and spindle shaped cells

productos

Not specified

receptores

Not specified

técnicas

cell culture | mammalian: suitable

enfermedades relevantes

cancer

Condiciones de envío

dry ice

temp. de almacenamiento

−196°C

Categorías relacionadas

Origen línea celular

Human adenocarcinoma

Descripción línea celular

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

Aplicación

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.
Tumourigenicity studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Seed cells at 2-4 x 10,000 cells/cm2; 5% CO2; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

Otras notas

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Artículos

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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