Nthy-ts1

90011610, human thyroid, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: Nthy-ts1, 90011610
• biological source: human thyroid
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human thyroid follicular mutant epithelial

Cell Line Description

Normal primary cultures of human thyroid follicular cells were transfected with a plasmid containing the SV40 early region large T encoding gene from mutant tsA58. Tight temperature dependence for growth is observed: Nthy-ts1 cells grow at 33°C, but not at the restrictive temperature of 40.5°C. This SV40 mutant allows the study of oncogene action free of any interfering effects of SV40 large T antigen.

Application

Transfection, mutation and virus studies: SV40

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 33°C.

Other Notes

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