Fao Cell Line from rat

89042701, rat liver, Hepatocyte

• UNSPSC Code: 41106514

Propiedades

• product name: Fao Cell Line from rat, 89042701, rat hepatoma
• biological source: rat liver
• growth mode: Adherent
• karyotype: Not specified
• morphology: Hepatocyte
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Rat hepatoma

Cell Line Description

A differentiated cell line derived from H4-11-E-C3 (Sigma catalogue no. 85061112). A versatile tool for studying regulation of mono-oxygenase activities and analysing their role in the activation of xenobiotics e.g. carcinogens, drugs and pesticides.

Application

Differentiation and enzymatic studies

Culture Medium

Coon′s modified Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or kaign′s modified Ham′s F12 + 45mg/l asorbic acid +18mg/1 inositol + 10% FBS +2mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂ 37°C.

Other Notes

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