BRIP human cell line

88052044, IHW Number: 9044, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human blood
• envase: tube of 5 μg 88052044-DNA-5UG; pkg of vial of cells 88052044-1VL
• etnicidad: Italian
• género: male
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice

Descripción

Descripción general

Consanguineous: Yes

Acciones bioquímicas o fisiológicas

A: 2402
Cw: 0701 1504
B: 5101 1517
HLA DRB1: 1101
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPB1: 0201 0402

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Nota de preparación

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

HLA A: 24
HLA A ief: 24.1
HLA B: 51 63
HLA B ief: 51 15.1
Bw4/bw6: 4
HLA Dw: 5
HLA DR: 11
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 2

Cláusula de descargo de responsabilidad

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.