VERO Cell Line

84113001, African green monkey kidney, Fibroblast-like

• Sinónimos: Vero 81 Cells, VeroCCL81 Cells
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: VERO Cell Line, 84113001, from African green monkey kidney, A fibroblast-like kidney cell, non-WHO origin, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• origen biológico: African green monkey kidney
• modo de crecimiento: Adherent
• cariotipo: 2n = 60, modal no. 58
• morfología: Fibroblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

Vero cells are anchorage-dependent cells and have wide applications in molecular and cell biology research. Vero cells have a pseudo-diploid karyotype and are preferred cell lines for the propagation of various viruses, including poliovirus, rotavirus influenza and rabies virus. These cells are useful hosts for vaccine production.

Origen línea celular

Monkey African Green kidney

Descripción línea celular

Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.

Aplicación

VERO cell lines have been used:

• in the propagation of Chandipura virus (CHPV)
• in the cytotoxicity assay to evaluate the antioxidant and anti-viral activities of Capsicum annuum methanolic extracts
• for the propagation of T.cruzi

Virus studies: polio, rubella, arboviruses and reoviruses

Medio de cultivo

(D6546) + 2 mM (G7513) + 10% (F2442).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Otras notas

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